June 30, 2022

Alkaline stability of Fc and VH3 binding in protein A resins

In the development of antibody purification protocols, you need to know your Ab molecule and its possible affinity binding sites for the chromatography resins. In this study, we have looked at how different antibody binding sites of a protein A ligand are affected by cleaning in place (CIP). We evaluated NaOH incubation and the effect of performed CIP cycles for two established protein A resins on the market.


Tags: cleaning, NaOH, alkaline stability, capture, chromatography, resin, protein A