May 24, 2018

Chromatography Scale-Up: Don’t Get Tied Down by Bed Height

chromatography scale upClick here to read the article >>  

When scaling, you can make better use of existing chromatography columns while avoiding overcapacity. 

Keeping a constant residence time is a well-established strategy when scaling up a chromatography step. Typically, this is achieved by keeping the bed height and linear flow velocity constant. But aiming for a constant bed height throughout scales has a limitation—you need an exact chromatography column size that fits the purpose. 

Finding the right column size can be tricky. The article below presents an alternative approach comprised of standardizing across scales to constant column volumes per hour (CV/h).

Tags: affinity chromatography, chromatography, microbial contamination, sanitization, cleaning, bioburden, monoclonal antibody, Mab, Protein A chromatography, protein A