May 24, 2018

Chromatography Scale-Up: Don’t Get Tied Down by Bed Height


chromatography scale upWhen scaling, you can make better use of existing chromatography columns while avoiding overcapacity. 

Keeping a constant residence time is a well-established strategy when scaling up a chromatography step. Typically, this is achieved by keeping the bed height and linear flow velocity constant. But aiming for a constant bed height throughout scales has a limitation—you need an exact chromatography column size that fits the purpose.

If the column is too small, you waste time by cycling it several times to reach the desired output. If the column is too big, you don’t use all the capacity you have and waste resin. Try an alternative approach: standardize across scales to constant column volumes per hour (CV/h) instead.

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