December 1, 2017
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
This work describes the capabilities of ÄKTA readyflux when used in ultrafiltration/diafiltration (UF/DF) applications. The process was conducted in an automated manner based on methods created using phases predefined in the UNICORN™ system control software. Concentration of 5 g/L bovine serum albumin (BSA) was performed using a 0.46 m2 filtration cassette with a Mr 10 000 nominal molecular weight cut‑off (NMWC) pore size, with process parameters fixed at a transmembrane pressure (TMP) of 1.2 bar and a feed flow of 4 L/min. Protein recovery was ≥ 99%, after 5‑times concentration and 5‑fold diafiltration. The process was performed according to set specifications for a tangential flow filtration (TFF) process, with the added benefits of the single‑use technology and consistency through automation.