March 23, 2017

Efficient concentration of a low-titer bovine IgG with high recovery in low volume

This application note describes the design of circuits with low working volume, allowing for high concentration of  dilute feed streams. The use of ReadyCircuit™ single-use assemblies allowed for aseptic connectivity of the filtration  device. Filtration was performed using ReadyToProcess™ hollow fiber filter cartridges. The described process enabled concentration of a bovine IgG antibody from 50.0 to 0.165 L in less than 5 h.

Tags: ReadyToProcess, hollow fiber cartridge, filtration, aseptic, single-use, bovine, IgG, concentration