To increase the yield of a target protein, fed-batch culture is often employed in biomanufacturing processes. This application note demonstrates the enhanced performance of commonly used cell lines for MAb production when supplemented with HyClone™ cell culture supplements. Cultures of Chinese hamster ovary (CHO) cells and the cholesterol-sensitive NS0 murine myeloma cell line were fed with HyClone Cell Boost™ nutrient supplements and HyClone LS1000 cholesterol supplement, respectively. Cell growth and MAb production in the fed-batch cultures were compared with batch cultures. The results demonstrate increased viable cell yields and productivity in the fed-batch cultures.
December 17, 2015