September 27, 2018

How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins

dynamic binding capacityIn protein purification, dynamic binding capacity (DBC) of a chromatography column describes the maximum amount of target protein that you can load onto your column without causing unnecessary loss, measured under realistic experimental conditions (default flow-rate, real protein sample). DBC is available in the technical specifications for GE columns and resins, for typical proteins and running conditions. This is good reference data; however, you might need to determine the DBC yourself to screen for the best resin and running conditions for your specific protein.

Tags: column, resin, DBC, dynamic binding capacity, chromatography, protein purification