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Perfusion processes have many advantages such as being favorable for product quality and supporting efficient facility utilization by offering the possibility of using smaller bioreactors. Compared with batch or fed-batch processes, perfusion processes also allow cells to be maintained in exponential growth phase for an extended period of time and reach higher viable cell densities.
The work demonstrates perfusion culture of an antibody producing Chinese hamster ovary (CHO) cell line, using either tangential flow filtration (TFF) or alternating tangential flow filtration (ATF) as cell retention method.