The new Protein A fiber chromatography format allows for quick purification of many samples because of its short cycle times. This makes the pre-packed, small-scale Protein A fiber unit a versatile tool for processes such as clone selection and upstream cell-culture media development. The time savings in downstream processes are also significant. Screenings of different buffer conditions and lifetime studies, including hundreds of cycles, can be performed in less than 24 hours. On the large scale, the Protein A fiber operated in a rapid cycling manner allows full utilization of the Protein A lifetime during one batch.
In this webinar, examples will be given from small-scale, where automation and a combination of capture and desalting steps will increase the throughput in upstream process development, as well as buffer and CIP optimization in downstream process development. The compatibility of fiber units with current chromatography systems regarding system flow rates, back pressure limitations, and dead volumes will also be demonstrated. Productivity, throughput, buffer consumption, and purification performance will be compared to traditional Protein A column chromatography.
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