January 16, 2020

Scalable ADCF Virus Production Using HyClone™ VaccineXpress Medium

In this study, HyClone VaccineXpress cell culture medium was used for the propagation of influenza virus and rotavirus in an animal-derived component-free (ADCF) process. Small-scale microcarrier cultures were optimized using spinner flasks by closely monitoring cell growth, viability, and morphology. The results were used for scaling up the process to a ReadyToProcess WAVE 25 bioreactor system at a 2-L working volume. The data show that the ADCF process using VaccineXpress for rotavirus propagation is scalable, promoting the production of infectious viral particles.

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Tags: VaccineXpress, Wave, microcarrier, medium, cell culture, vaccines, Hyclone