November 17, 2017

Single-Use Workflow for Recovery of a Domain Antibody from E. coli Culture Feed in an Automated Manner

AKTAThis application note demonstrates the performance of the automated ÄKTA™ readyflux single-use filtration system in microfiltration applications. Here, a domain antibody (dAb) expressed in E. coli was recovered from fermentation broth. The dAb-containing sample was clarified (2.5-times concentration) and further washed with three wash volumes, with a recovery of approximately 90% of product in the permeate.

Tags: ÄKTA readyflux, cell culture, e.coli, hollow fiber, TFF, domain antibody, microfiltration, single-use, Recovery