May 18, 2014

The use of Capto™ Core 700 and Capto Q ImpRes in the purification of human papilloma virus like particles

This application note describes a purification procedure for human papillomavirus (HPV) L1 protein, the antigenic component of HPV vaccines. Modified L1 protein was expressed in insect Sf9 cells using baculovirus vector, and allowed to spontaneously assemble into virus like particles (VLPs). A modern, scalable approach, based on two chromatographic steps including novel chromatography media (resins), was used for the purification of L1 protein. In the first step, VLPs are purified in flow-through mode using Capto Core 700, a layered-bead size exclusion medium. In the second step, Capto Q ImpRes medium allows polishing of the L1 protein with high purity and resolution.

Tags: chromatography; bioprocess development, orthogonal separation, bind/elute, flow-through, HPV, Capto