September 23, 2013

The use of Xcellerex™ mixing system as slurry tank when packing chromatography columns

This application note describes the performance of single-use Xcellerex XDM Quad Mixing System used as a slurry tank when packing chromatography columns.  The capability of the mixing system to keep the chromatography medium (resin) in suspension and the possibility to integrate the system in column packing were studied. The results show that XDM Quad Mixing System is suitable for use as a media slurry tank when packing chromatography columns.

Xcellerex XDM Quad Mixing System is designed for convenient and efficient mixing of buffer, media, product, and other process fluids (Fig 1). The single-use system eliminates the hassle of time-consuming and costly steamin-place, clean-in-place, and cleaning validation procedures.  The result is an efficient processing and rapid batch-to-batch turnover time.

The XDM Quad Mixing System comprises a full-featured, integrated, and rigid containment system. The system is complete with motor and controls, and an irradiated USP Class VI, single-use, low-density polyethylene plastic bag equipped with a disposable bottom-mounted impeller. The connection between motor and disposable impeller is via a robust magnetic coupling. The agitation is driven through stainless steel bearings within the impeller, providing high torque and rapid mixing to the system.  

DEAE Sepharose™ Fast Flow chromatography medium was used to investigate the ability of XDM Quad Mixing System to keep the medium in suspension. The chromatography medium was mixed for one hour before the bag was drained and media sedimentation was monitored. In a related study, the XDM Quad Mixing System was evaluated for use with an AxiChrom™ 300 chromatography column. The mixer was used with the Intelligent Packing concept of AxiChrom columns for packing Capto™ adhere ImpRes.

Tags: mixing, disposable, single-use, packing, column, chromatography, Xcellerex