Are you frustrated that you aren’t getting the amount of pure protein that you expected? Don’t worry!
For example, if your protein recovery is higher than expected, it could be caused by proteins coeluting with other substances. If you are experiencing poor binding, it’s possible that your sample has the wrong pH. Maybe your recovery is lower than expected, which could mean the protein is being degraded by proteases.
Read other common reasons for protein recovery issues, and how to fix them, here.