July 2, 2019
Animal Component-Free Rotavirus Production Using Microcarriers
Rotavirus (RV) disease is a significant cause of diarrhea in children and a serious issue in developing countries. There are a number of RV vaccines on the market that are primarily produced using static cell culture in a serum-containing medium. Furthermore, porcine trypsin is typically used for virus activation. Here we present a method in which RV can be propagated in Vero cells expanded on microcarriers using recombinant trypsin and a serum-free cell culture medium. The results demonstrate that RV can be produced using animal origin-free raw materials to meet regulatory requirements. Additionally, the scalability of this method has the potential to significantly improve the productivity and economy of industrial RV vaccine production.