November 16, 2011

Current advances in Protein A chromatography

In MAb purification processes Protein A media (resins) are often used for the capture step because they produce high purity and yield after a single chromatography step. The increasing demand for MAbs as biopharmaceuticals has promoted the development of cell cultures with increased expression levels. As a consequence, the need for a more efficient capture step has increased. In this work we present results with a novel high capacity Protein A medium prototype based on high flow agarose. 

The main factors that influence dynamic binding capacity (DBC) are:

· ligand density

· particle size

· accessible surface area

Sub-optimization of one parameter often results in decreased performance in something else. Thus, thorough optimization is needed.

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