November 5, 2015

Guidelines for protein purification at low temperature


The main reason for performing protein purification at low temperature is to preserve the integrity of proteins and whole samples during the process. Working at lower temperature reduces proteolytic activity and prevents unwanted degradation of the target protein. The tendency of the target protein to aggregate should also be monitored and can be impacted by factors such as temperature and protein concentration. There are many factors to consider when working with proteins at low temperature. This document will provide you with general guidelines on how to successfully purify proteins under such conditions.

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