In a biopharmaceutical plant, more that 50% of the operation is mixing or hydration. Single-use mixers have been used in the past two decades in biopharmaceutical plants to replace the use of stainless steel vessels. Heating/cooling and mixing properties are the two key parameters important for mixing applications and are needed when comparing the performance between stainless-steel and single-use mixers. In this study, two parameter—heating/cooling and liquid/liquid mixing time—were characterized for a range of volumes and impeller speeds in the 50, 200, and 500 L XDM or XDUO mixers.