When running small-scale size exclusion chromatography (SEC) it is important to keep the internal volume in the chromatography system as low as possible for optimal performance. Tubing and components shall be selected with the internal volume in mind. The cue card Optimal configuration of ÄKTA™ pure 25 for small-scale SEC describes how to improve results when performing SEC with sample volumes from 10 μl to 500 μl for analysis or small-scale preparative purification. The cue card explains how to configure the ÄKTA pure and recommendations for what components to use.
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