June 17, 2013

Preparation and High Resolution Analysis of a Fab


MAb fragments are getting increased attention in research use due to their small size and lower immunogenicity (relative to intact antibodies).

In this work, Fab was produced by papain cleavage of a purified monoclonal antibody (MAb) followed by a purification on MabSelect Sure™ and Capto™ L media (Protein A and Protein L immobilized to high flow agarose, respectively).

Fractions from the different steps were analyzed with a new size exclusion chromatography (SEC) medium, Superdex™ 200 Increase.

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