April 5, 2017

Setting new standards for cleaning and sanitization of Protein A chromatography resins


The aim of this work was to study molecular modifications of existing and pipeline protein A affinity ligands after extensive exposure to commonly used and new cleaning and sanitization agents. Amino acid site specific modifications were elucidated by high resolution mass spectrometry (MS) and the effect on kinetics and binding affinity was monitored by surface plasmon resonance (SPR). Finally, the functionality and lifetime of the protein A resins after regular exposure to cleaning and sanitization agents were measured by confocal microscopy and conventional chromatographic methods.

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