January 31, 2019

Single-Step, Protein A Chromatography Process for Bispecific Antibodies in Early Screening


A single‑step downstream process using protein A chromatography to purify a bispecific antibody (BsAb) in early screening has been successfully developed to replace an existing‑three step process. The goal was to separate BsAb monomer from fragments such as the individual heavy and light chain pairs while achieving high purity and yield of BsAb. MabSelect™ PrismA protein A resin was selected for the capture step. Most fragments could be removed as early as the capture step using gradient elution from pH 5 to 3.5, without the need for additional purification steps. BsAb constructs were efficiently purified to give more than 90% purity and 85% yield in the early screening downstream process.

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