December 3, 2014

Utilization of high-throughput screening in the development of HyCell™ CHO medium


The nutritional requirements of the Chinese hamster ovary (CHO) cell line vary specific to the individual recombinant clones isolated, often necessitating the development of a clone-specific production medium to achieve optimal productivity in a bioprocess. Traditionally, such medium development efforts have primarily incorporated shake flask cultures, which limit the number of conditions, the number of replicates, and the ability to incorporate advanced design of experiments (DoE) statistical methods. In order to overcome these limitations, this poster investigates the use of various high throughput screening (HTS) equipment in the development and testing of a medium designed for multiple CHO clones. HTS allowed for the inclusion of more individual cell lines, increased component factor-level and mixture design treatments, and all with a higher number of replicates yet less labor-intensive methods.

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