Search results for " DNA" in Articles / App Notes

Article Scaling Up an E. coli Upstream Process for Plasmid DNA Production — from 2.5 to 160 L scale Fermenters
coli growth measured as optical density at 600 nm (OD600), plasmid titer, and percentage of produced supercoiled (SC) plasmid DNA was comparable at all culture volumes tested. Explore th…

Article Purification of Plasmid DNA
By switching to modern chromatography resins, Cobra Biologics was able to increase binding capacity and flow rates up to 50% to address the increasing demands for high quality plasmid DNA. Click h…

Article Akron Bio Will Install a Cytiva FlexFactory to Manufacture Plasmid DNA
Cytiva will provide its FlexFactory, a flexible, single-use platform, to enable Akron Biotech to manufacture plasmid DNA (pDNA), which is used in many innovative therapies and vaccines, Cytiva said in…

Article Improving Capacity in DNA Plasmid Purification
Working with plasmids? Plasmid supply is in high demand these days. Join Tony Hitchcock on May 6, 2020 to learn more about this critical market and how Cobra Biologics is working with Cytiva …

Article Managing Residual Impurities During Downstream Processing
Host-cell DNA will also be present and must be minimized by the purification process. Elemental impurities can be present as well. There is, however, no set of standard assays for the determination of…

Article New Therapies Present Scaling Challenges
One major challenge to a scale-up strategy lies in the crucial step of transient transfection, which is when DNA is introduced into the mammalian cell. The genetic material to be introduced into the m…

Article Genetic Vaccine Platforms Demonstrate Their Potential
Genetic vaccines use part of the virus’ own genetic code (either mRNA or DNA) to instruct cells to make a viral antigen that will stimulate an immune response. Viral-vectored vaccines allow selection …

Article Novel Vaccine Technologies Meet the Need for Pandemic and Therapeutic Solutions
Examples include cell-culture, synthetic DNA, chimeric antigen, and recombinant protein nanoparticle vaccine technologies. Egg-vaccine technology limitations Traditional vaccine manufactu…

Article Best Practices for Selecting a Top-Quality Cell Line
“Equally important,” stresses Fisch, “is the quality of the recombinant DNA expression and transfection systems. Incorporating recombinant DNA stably into a cell line’s genome in a manner that promote…

Article Development of Purification for Challenging Fc-Fusion Proteins
As tested on three Fc-fusion proteins, this process demonstrates high process yield, improved clearance of HMWs and LMWs, and efficient reduction of host cell proteins (HCP) and DNA as well as robust …

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