Search results for " size exclusion"

Article Selection Guide Size Exclusion Chromatography Columns and Media
Size exclusion chromatography (SEC), also called Gel filtration (GF), separates molecules on the basis of differences in size as they pass through a SEC medium packed in a column. A variety of media…

Article Selectivity in Size Exclusion Chromatography (SEC): It’s Much More than Average Pore Diameter (Å)
Describing the pore of a size exclusion chromatography resin with a single number such as average pore diameter is an oversimplification. The formation of pores is dependent on complex combinations of…

Article Protein Analysis with Size Exclusion Chromatography
Size exclusion chromatography (SEC) is widely used in several different analytical applications from basic research to quality control of biotherapeutics. This white paper presents fundamentals abou…

Article Sephadex™ Size Exclusion Resin Turns 60
Do you know the story behind Sephadex, which enabled today's large-scale production of biopharmaceuticals? An inadvertent discovery in 1959 became the foundation for today's biopharma industry. This i…

Article Handbook: Size Exclusion Chromatography Principles and Methods
This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection crite…

Article HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Many of the recommended protein characterization assays are based on liquid chromatography methods such as ion exchange chromatography (IEC) for charge heterogeneity analysis, size exclusion chromatog…

Article SEC in the Modern Downstream Purification Process
Mar 01, 2015 By R. Christopher Manzari, J. Kevin O'Donnell BioPharm International Volume 3, Issue 28 Since the introduction of commercial chromatography resins approximately six de…

Article Virus-like Particles as Therapeutic Moieties of the Future
Further, aggregation and degradation of VLPs can be studied by AFM, cryo-EM, DLS, interlaced size exclusion–ultra high performance liquid chromatography, IVRP assay, MS, native agarose gel electrophor…

Article Analyzing Proteins Using SEC, MALS, and UHPLC
Light scattering analysis combined with more rapid size exclusion chromatography improves protein characterization. The characterization of proteins involves identification of their complex …

Article Process Chromatography Selection for Downstream Processing Applications
…ductivity, since the binding of impurities is primarily due to van der Waals interactions as well as size exclusion. Membrane adsorbers have been available for more than a decade, and while their…

Next Page