Search results for " hydrophobic interaction"
Article
Fundamentals of hydrophobic interaction chromatography
Hydrophobic interaction chromatography (HIC) is a versatile technique that can be used for capture, intermediate purification, or polishing steps.
This technique separates proteins accor…
Article
New hydrophobic interaction chromatography (HIC) handbook
Hydrophobic interaction chromatography (HIC) separates biomolecules under mild conditions according to differences in molecular hydrophobicity. HIC is often used as a next step to purify prote…
Article
Fundamentals of Hydrophobic Interaction Chromatography
This article explores the fundamentals and applications of hydrophobic interaction chromatography using Capto™ resins to increase productivity.
Click here to read the article.
Article
Increasing Productivity in Hydrophobic Interaction Chromatography (HIC) Using Capto™ Resins
In this study, the hydrophobicities of newer-generation Capto™ HIC resins were compared to Sepharose™ High Performance and Sepharose Fast Flow HIC resins. An application example is also present…
Article
Evolution of the Monoclonal Antibody Purification Platform
These resins combine different types of interactions such as ionic interaction, hydrogen bonding, and hydrophobic interaction. This can result in a different selectivity than what is offered by tradit…
Article
Process Chromatography: Continuous Optimization
Improved resin chemistries and customized separation solutions are enabling more efficient separations.
By Cynthia A. Challener
Cell-culture and fermentation are high-…
Article
Challenges of Protein Aggregation During Purification
At the same time, strong hydrophobic interactions between the resin and the product may lead to aggregate formation, according to Vedantham, again due to surface-induced structural perturbation of the…
Article
Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
The necessity to detach cells from a culture substrate during cell harvesting remains one of the most challenging steps in a cell-culture process.
By Marcos Simon, Juan J. Giner-Casares
Cell…
Article
Development of Purification for Challenging Fc-Fusion Proteins
…olishing process that incorporates flow-through strategy in polishing steps using cation exchange or hydrophobic interaction chromatography.
As tested on three Fc-fusion proteins, this p…
Article
SEC in the Modern Downstream Purification Process
Biomolecules typically should not bind to an SEC resin like they do in ion-exchange chromatography, affinity chromatography, hydrophobic interaction, or mixed-mode chromatography. As a result, mobile-…