Search results for " anion exchange"

Poster Mab polishing using a new multimodal anion exchanger
To address theses challenges, a new multimodal anion exchange medium with a particle size designed for polishing (40 mm) was developed. The new medium, Capto™ adhere ImpRes, displays high resolution, …

Article Purification Strategies to Process 5 g/L Titers of Monoclonal Antibodies
Depending on the platform, this is followed by either the cation exchange chromatography step in bind-and-elute mode3 or the anion exchange chromatography step in flow-through mode. The virus filtrati…

Article Evolution of the Monoclonal Antibody Purification Platform
While both the antibody and impurities bind to the anion exchange resin, the impurities are much more tightly bound than in flow-through mode resulting in significantly higher impurity clearance. Load…

Article The Development and Application of a Monoclonal Antibody Purification Platform
After cation exchange, diafiltration was performed to prepare the product for anion exchange chromatography in flow through mode, in which impurities such as DNA and some HCPs were captured on the res…

Article Developing a mAb aggregate removal step by high throughput process development
…nd for the aggregates (Qa) can be calculated according to the following equation: For the strong anion exchanger (Capto Q), the highest capacity for both monomer and aggregates was observed at hi…

Article Development of Purification for Challenging Fc-Fusion Proteins
Development of Purification for Challenging Fc-Fusion Proteins This study outlines methods for an alternative protein-polishing process for challenging proteins. …

Article Process Chromatography: Continuous Optimization
Polishing is then achieved using some combination of anion exchange (AEX), cation exchange (CEX), mixed mode, and HIC resins. “Protein-A based affinity chromatography is very popular and more wide…

Article Bioterminology: A Guide to the Biopharmaceutical Lexicon
It is an anion exchange resin.  deamidation Removal of one or more amide groups from the Gln or Asn residue in a protein, converting the residues to Glu, Asp, or isoAsp. Depending on the protein,…

Article Mixed-Mode Chromatography in Downstream Process Development
In contrast, carboxylic side chains of proteins bind to the Ca2+ ions of the resin by a combination of calcium metal affinity and anion exchange. The combination of different binding modes leads to ex…

Article Polishing of monoclonal antibodies using Capto adhere ImpRes in bind and elute mode
Capto adhere ImpRes is a multimodal anion exchange medium with a ligand (Fig 1) that displays high selectivity compared with traditional ion exchange polishing media. The medium enables operation in e…

Next Page