Search results for " DBC"

Article How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins
This is good reference data; however, you might need to determine the DBC yourself to screen for the best resin and running conditions for your specific protein. Learn more about how to determine …

Article Polishing of monoclonal antibodies using Capto adhere ImpRes in bind and elute mode
Results and discussion Case study, MAb A The case study with MAb A shows a suggested workflow for method development including screening of conditions for SBC and DBC, screening of elution con…

Article Purification Strategies to Process 5 g/L Titers of Monoclonal Antibodies
If the DBC can be doubled to 30 g/L, then the maximum recoverable titer increases to 3 g/L and a DBC of 45 g/L accommodates titers of up to 5 g/L. An increase in DBC can be achieved in several ways: b…

Article Polishing of Monoclonal Antibodies using Capto™ MMC ImpRes in Bind and Elute Mode
The trends seen in the PreDictor plate SBC experiment correlated well with the DBC studies (Fig 5). The condition with highest DBC (100 mM NaCl, pH 6.0) was chosen for further investigation in which t…

Article Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The result of process optimization was a DBC of >100 mg mAb/mL medium at a loading condition of pH 5.0 and 50 mM NaCl. A Tricorn 5/100 column (GE Healthcare) was used to verify the DBC at 10% breakthr…

Article Comparing Protein A Resins for Monoclonal Antibody Purification
According to the vendor, Resin 3 exhibits higher DBC than Resin 2 at longer residence times (8). A residence time of 6 min is expected to give a DBC (at 10% breakthrough) of approximately 60 g antibod…

Article A platform approach for the purification of antibody fragments (Fabs)
For instance, the load was 15 mg/ml, which corresponds to 70% of the DBC, a representative load for a production scenario. The residence time during the load was 4 min and the wash volume was 7 column…

Article Re-use of Protein A Resin: Fouling and Economics
Approximately 80% of industrial respondents have observed a decrease in DBC and flattening of the slope of the breakthrough curve as the cycle number increases, particularly with MabSelect SuRe. For o…

Article Current advances in Protein A chromatography
MabSelect SuRe LX and MabSelect SuRe DBC were also compared for seven different MAb-containing feedstocks. At longer residence times, MabSelect SuRe LX displayed 20% to 46% higher DBC. The productivit…

Article Capture of human single-chain Fv (scFv) fusion protein on Capto L affinity medium
Results shown in Figure 7 indicate a DBC of 23 mg/mL at 10% breakthrough. Following optimization of the wash and elution steps and determination of DBC, two consecutive runs were perfor…

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