Search results for " aggregation"

Article Detecting Protein Aggregates and Evaluating their Immunogenicity
Protein aggregation can occur during all stages of the lifetime of a protein therapeutic, including expression, refolding, purification, sterilization, shipping, storage, and delivery processes (1). T…

Article Challenges of Protein Aggregation During Purification
…increased as titers have increased, if unsuitable conditions are selected for downstream operations, aggregation can also occur during downstream processing. Aggregation mechanisms There are …

Article Predicting Progress in Protein Aggregation
As the development of biologic drugs based on antibodies and other protein/peptide molecules continues apace, the control and prevention of aggregation during manufacturing, downstream processing, for…

Article Developing a mAb aggregate removal step by high throughput process development
High throughput process development allows rapid screening of chromatographic parameters. By Gustav Rodrigo and Kristina Nilsson-Välimaa ABSTRACT This article presents a case study ill…

Poster Rapid screening of antibody aggregates and protein impurities using short gel filtration columns
Purification of antibodies often generates dimers and higher aggregated forms and during optimization of purification protocols, many samples may need to be analyzed for aggregate content.  Gel f…

Poster Optimizing dynamic binding capacity and aggregate clearance in an mAb polishing step
Capto™ S ImpAct is a strong cation exchange (CIEX) chromatography medium (resin) designed for monoclonal antibody (MAb) polishing steps. In this study, optimization of binding conditions targeting h…

Article Modeling the Degradation of mAb Therapeutics
Both physical effects such as aggregation and chemical modifications (e.g., fragmentation) are the two leading paths for mAb degradation. Kinetic modeling can be an effective tool for establishing pro…

Article Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
Nesta Abstract The objective of this study was to assess the impact of manufacturing-scale, freeze-thaw conditions on aggregation and subvisible particle formation of a monoclonal antibody sol…

Article Polishing of Monoclonal Antibodies using Capto™ MMC ImpRes in Bind and Elute Mode
A rapid procedure to establish a robust second step in bind/elute (B/E) mode for the purification of a MAb using Capto MMC ImpRes has been developed. This study presents results from optimization …

Article Polishing of monoclonal antibodies using Capto adhere ImpRes in bind and elute mode
This application note describes a study where the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute (B/E) mode was evaluated. For a …

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