Search results for "host cell protein"

Article A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
The authors describe the ways in which manufacturers can mitigate the risks related to the integrity of recombinant transgenes expressed in CHO cells. By Luhong He, Christopher Frye Abstract …

Article Scale-up of adherent Vero cells grown on Cytodex microcarrierrs using ReadyToProcess equipment
In cell culture-based vaccine production, scale-up of adherent cells is challenging. This study shows a process for scaling up adherent Vero cells from static cell factories to influenza producti…

Article Implications of Cell Culture Conditions on Protein Glycosylation
The authors present a review of the techniques commonly used for glycosylation analysis. By Michiel E. Ultee, PhD, Dr. Richard Easton This article reviews the implications of cell-culture co…

Poster Host Cell Proteins beyond ELISA with label free interaction and imaging technologies
This technology was used to characterize the effect of different culture conditions on MAb expressing CHO cells in terms of almost 1700 individual host cell protein profiles and overall patterns in ha…

Poster Sensitive methods for evaluation of antibodies for host cell protein analysis and screening of impurities in a vaccine process
An evaluation of the affinity spectra of HCP antibodies purified from rabbits immunized with Vero cell lysate, using two different adjuvants: FCA and AbISCO®.

Article Bioterminology: A Guide to the Biopharmaceutical Lexicon
Host cells contain hundreds to thousands of host cell proteins and other biomolecules that could contaminate the final product.  HPLC High-performance liquid chromatography or high-pressure liquid…

Article Impact of Media Components on CQAs of Monoclonal Antibodies
The authors review how media components modulate the quality of monoclonal antibody products.  By Anurag Rathore, Rajinder Kaur, Dipankar Borgayari  Recombinant protein products have…

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Article Addressing the challenges in downstream processing today and tomorrow
In addition, the HIC step reduced the level of host cell proteins by 1.4 log10 . Based on these data, the final process used a Protein A capture step with elution at low pH followed by immediate neutr…

Article Host-Cell Protein Measurement and Control
…ry scale, to demonstrate the removal of cell substrate-derived impurities such as nucleic acids and host cell proteins may sometimes be used to eliminate the need for establishing acceptance criteria…

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