Search results for " Predictor"

Article High-throughput screening of HIC media in PreDictor™ plates for capturing recombinant Green Fluorescent Protein from E. coli
Hydrophobic interaction chromatography (HIC) is a powerful purification technique where the type and density of the ligand, pH and salt of binding conditions, temperature and the nature of the targe…

Multimedia PreDictor™ plates and Assist software
Using PreDictor™ plates and Assist software helps to increase the efficiency of process development.

Poster High-throughput screening of HIC media in PreDictor™ plates for capturing a recombinant protein from E.coli
Hydrophobic interaction chromatography (HIC) is a powerful purification technique where the type and density of the ligand, pH and salt of binding conditions, temperature and the nature of the targe…

Article High-throughput process development and scale-up of an intermediate purification step for recombinant insulin
Conditions defined using PreDictor plates can be verified and optimized in column format with HiScreen™ or HiTrap™ prepacked columns. An automated chromatography system such as AKTA avant 25 wil…

Article High-Throughput Process Development Handbook
This handbook focuses on process development work in PreDictor 96-well filter plates, which are prefilled with chromatography media (resins). The handbook describes how results obtained using PreDicto…

Tip Select Your PD Tools
For example, PreDictor™ 96-well filter plates is a good choice for early screening of chromatography media (resin) or for initial screening of a wide range of operating conditions, with very limited s…

Article Capture of human single-chain Fv (scFv) fusion protein on Capto L affinity medium
We describe the capture of a single-chain Fv fusion proteinfrom a challenging feedstock (animal plasma) on the affinitychromatography medium (resin) Capto™ L. Initial work on smallprepacked HiScreen™ …

Article High-throughput screening and process development for capture of recombinant pro-insulin from E. coli
Screening in PreDictor plates also saved considerable time and sample compared with column experiments. A second study describes a similar workflow for the intermediate purification of the insulin obt…

Article Polishing of Monoclonal Antibodies using Capto™ MMC ImpRes in Bind and Elute Mode
Determination of static binding capacity Static binding capacity (SBC) was determined in 6 μL PreDictor™ Capto MMC ImpRes 96-well plates. Equilibration of wells in the plates was performed by addi…

Article Miniaturization to Become Even More Successful
Several talks focused on the use of miniaturized columns and screening plates, such as PreDictor 96-well plates and PreDictor RoboColumn™ units, and the possibility of these formats to become even mor…

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