Search results for "western blotting"
Western Blot Detection: Is Film or a Digital CCD Imager Better?
Learn the benefits and drawbacks of using film or a CCD imager to detect the bound antibody in Western blotting in this blog post. Learn more
Western blotting principles and methods
A new handbook intended to guide beginners as well as experts toward successful Western blotting is available here.
Bioterminology: A Guide to the Biopharmaceutical Lexicon
Bioterminology: A Guide to the Biopharmaceutical Lexicon This lexicon was generated by the editors of BioPharm International magazine. Please feel free to email your suggestions for terms that s…
Host-Cell Protein Measurement and Control
Jun 01, 2015 By Fengqiang Wang, PhD, Daisy Richardson, Mohammed Shameem BioPharm International Volume 28, Issue 6, pg 32–38 Host-cell proteins (HCPs) constitute a major part of proce…
Impurity Testing of Biologic Drug Products
Experts share insights on the various methods used for purity and impurity analysis of therapeutic proteins. By Adeline Siew, PhD CA-SSIS/shutterstock.comIm…
Managing Residual Impurities During Downstream Processing
Increased understanding of potential impurities has spurred efforts to standardize monitoring procedures. By Cynthia A. Challener Manufacture of protein and other biologic drug substances via …
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and Western blotting demonstrate protein integrity and in the case of antibodies, the presence of heavy-heavy-light, heavy-light, o…
Accurate comparability assessment of a biosimilar interferon in process development
This application note describes how to achieve accurate comparability assessments using Biacore™ T200 and Amersham™ WB system in the development of a biosimilar. This is exemplified in this study…
Purification of alpha-1 antitrypsin using an antibody based affinity chromatography medium
A new method for purification of alpha-1 antitrypsin (AAT) is presented. An agarose matrix functionalized with recombinant single domain antibody fragments recognizing AAT was shown useful for ca…