Search results for " gel filtration"

Poster Rapid screening of antibody aggregates and protein impurities using short gel filtration columns
Purification of antibodies often generates dimers and higher aggregated forms and during optimization of purification protocols, many samples may need to be analyzed for aggregate content.  Gel f…

Article Bioterminology: A Guide to the Biopharmaceutical Lexicon
Bioterminology: A Guide to the Biopharmaceutical Lexicon This lexicon was generated by the editors of BioPharm International magazine. Please feel free to email your suggestions for terms that s…

Article Virus purification using superheated buffer in place (SBIP) for packed bed chromatography
We have verified that a range of gel filtration (GF; also known as size exclusion) and ion exchange (IEX) chromatography media maintain performance and functionality after sterilization using SBIP. Pr…

Article Downstream scale-up purification of influenza virus using ReadyToProcess equipment
This application note describes a case study where live influenza virus was purified using single-use ReadyToProcess technology. The ReadyToProcess platform is a flexible manufacturing solution, whi…

Article Faster scale-up of a two-step MAb process with AxiChrom™ columns and ReadyToProcess™ solutions
Analytical gel filtration data showed that the scaled-up process reduced the starting aggregate concentration of 12% to 0.6% in a single step. The 86% monomer yield is considered good for a starting s…

Article Polishing of monoclonal antibodies using Capto adhere ImpRes in bind and elute mode
Determination of aggregates and aggregate clearance Fractions from the chromatographic runs were collected and analyzed by gel filtration (analytical size exclusion chromatography) on a Superdex™ …

Tip Purification paradIgM
Core bead technology provides an opportunity to purify IgM on the basis of its molecular size while offering higher productivity than gel filtration. Capto™Core 700 has been tested for IgM purificatio…

Article Selection Guide Size Exclusion Chromatography Columns and Media
Size exclusion chromatography (SEC), also called Gel filtration (GF), separates molecules on the basis of differences in size as they pass through a SEC medium packed in a column. A variety of media…

Tip Teaching the Basics of Chromatography
The videos explain techniques such as hydrophobic interaction chromatography, ion exchange chromatography, gel filtration (size exclusion) chromatography, and affinity chromatography. These vide…

Article Polishing of Monoclonal Antibodies using Capto™ MMC ImpRes in Bind and Elute Mode
A rapid procedure to establish a robust second step in bind/elute (B/E) mode for the purification of a MAb using Capto MMC ImpRes has been developed. This study presents results from optimization …

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