Search results for "hcp"
Article
Challenging Host Cell Protein Assays for Improved Risk Mitigation
We will address how to challenge your assays early to ensure accurate measurements, build confidence, and reduce the risk of unexpected HCP levels.
Article
Host cell protein quantitation using ELISA
However, it’s challenging to detect HCP, and this step can be time-consuming.
This application note shows how using the sensitive HCPQuant™ CHO ELISA kit (Cytiva) and automating wash steps using Aqu…
Article
Re-use of Protein A Resin: Fouling and Economics
Specificity of Protein A ligand towards mAb results in extremely effective clearance of host cell proteins (HCP), viruses, and DNA (2–5). The high selectivity is accompanied by reasonably good physioc…
Article
Evolution of the Monoclonal Antibody Purification Platform
Though it may not be as efficient as Protein A in terms of selectivity of HCP, it can be used in combination with effective HCP precipitation step. Additionally, to gain similar HCP clearance compared…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
…, degradation products, processing variants) and cell-derived impurities such as host cell protein (HCP) and cellular DNA. In response, platform purification processes for MAbs, i.e., those designed …
Article
Current Trends in Host Cell Protein Detection for Biologics Manufacturing
Commonly accepted HCP analytical methods, the challenges of HCP assay, and recommendations on what can easily be accomplished in-house and when it may be better to outsource.
Read more here…
Poster
Comparison of Three Different Screening Techniques for Predicting Step Elution Conditions for a Cation Exchanger
The aim of this study was to investigate conditions for reduction of host cell proteins (HCP) on a strong cation exchanger, Capto™ S ImpAct. Three different screening techniques using PreDictor™ plate…
Article
Moving PAT from Concept to Reality
Numerous at-line analyses evaluate host cell proteins (HCPs), DNA, viable cell density, bioburden, aggregates, product concentration, and particulates.
One of the greatest difficulties with many c…
Article
Impurity Testing of Biologic Drug Products
For example, ELISA is the gold standard and work horse for host cell protein measurement, but it only measures the total HCP and can’t give detailed information on the level of each individual host ce…
Article
Comparing Protein A Resins for Monoclonal Antibody Purification
Table two
The results show that the step yield was consistently over 95%, and high log-reduction factors of host-cell proteins (HCP) and DNA were achieved (see Figure 1). In this study, car…