Search results for " affinity chromatography"
Article
A Closer Look at Affinity Ligands
Bioprocess scientists and engineers realized the importance of affinity chromatography in purification of mAbs in the early 1980s and identified a small, 42 kD protein (i.e., Protein A) that has excep…
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A Look at the Affinity Chromatography Landscape
Thermo Fisher Scientific has taken the approach of developing product-specific affinity chromatography ligands based on its camelid VHH antibody fragment technology, according to Laurens Sierkstra, af…
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Affinity Chromatography Handbook in 3 Volumes
The Affinity Chromatography Handbook from Cytiva is divided into three volumes:
Affinity Chromatography, Vol. 1: Antibodies
Affinity Chromatography, Vol. 2: Tagged Proteins
Affinity Chro…
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Webinar about a new affinity chromatography technology for recombinant proteins
Watch this webinar to learn more about this new affinity chromatography technology!
Who should watch this webinar?
• Scientists who are already working with tagged protein purification technol…
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Affinity chromatography Handbook - Vol. 3: Specific Groups of Biomolecules
Affinity chromatography (AC) separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. The techni…
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Antibody Fragments and Their Purification by Protein L Affinity Chromatography
In the case of recombinant full-length antibodies with suitable Fc regions, two or three column purification processes centered around Protein A affinity chromatography have proven to be fast, efficie…
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Efficient Cleaning-In-Place Methods for Protein-Based Antibody Affinity Chromatography Resins
This whitepaper discusses cleaning of affinity resins intended for use in the purification of monoclonal antibodies and antibody fragments, for example, Fab fragments. Various cleaning strategies and …
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Production of recombinant proteins using a novel affinity technology
This scientific paper from Journal of Chromatography A describes results of a study utilizing a Cytiva Protein Select™ affinity resin and tag platform to produce any recombinant proteins.
Re…
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Guide for selecting affinity resins for mAbs, bsAbs, and antibody fragments
Development of antibody-derived molecules are increasing. Today, molecules such as bispecific antibody (bsAb) and antibody fragments such as Fab, scFv, and VHH are seen in labs all over the w…
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Bind, Cut, Elute — Easy Affinity Tag and Protease Removal
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This blog post discusses automated methods for removing an affinity tag and the protease. The tag is cleaved while the protein is still bound to the affinity re…