Search results for " affinity chromatography"

Article A Look at the Affinity Chromatography Landscape
Thermo Fisher Scientific has taken the approach of developing product-specific affinity chromatography ligands based on its camelid VHH antibody fragment technology, according to Laurens Sierkstra, af…

Article Affinity Chromatography Handbook in 3 Volumes
The Affinity Chromatography Handbook from Cytiva is divided into three volumes: Affinity Chromatography, Vol. 1: Antibodies Affinity Chromatography, Vol. 2: Tagged Proteins Affinity Chro…

Article Affinity chromatography Handbook - Vol. 3: Specific Groups of Biomolecules
Affinity chromatography (AC) separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. The techni…

Article Antibody Fragments and Their Purification by Protein L Affinity Chromatography
In the case of recombinant full-length antibodies with suitable Fc regions, two or three column purification processes centered around Protein A affinity chromatography have proven to be fast, efficie…

Article Efficient Cleaning-In-Place Methods for Protein-Based Antibody Affinity Chromatography Resins
This whitepaper discusses cleaning of affinity resins intended for use in the purification of monoclonal antibodies and antibody fragments, for example, Fab fragments. Various cleaning strategies and …

Article Bind, Cut, Elute — Easy Affinity Tag and Protease Removal
CLICK HERE TO LEARN MORE >> This blog post discusses automated methods for removing an affinity tag and the protease. The tag is cleaved while the protein is still bound to the affinity re…

Article Affinity chromatography Handbook: Vol. 2 Tagged Proteins
This handbook is intended for those interested in the expression and purification of recombinant proteins. The use of recombinant proteins has increased greatly in recent years, as has the wealth of…

Article Evaluation of Performance of a Disposable mAb Affinity Chromatography Column Used Over Multiple Process Cycles
Presanitized and prequalified disposable chromatography columns are widely used to save time in downstream processes. However, reuse of such columns could positively impact process economy. This app…

Article Affinity Chromatography Handbook: Vol. 1 Antibodies
The diversity of the antibody-antigen interaction and our ability to manipulate the characteristics of the interaction has created many uses for antibodies and antibody fragments, both for immunoche…

Article Case Study: Virus Reduction of an Affinity Capture Step
Evaluating how much a process step contributes to viral clearance is an essential part of process validation. For this reason, there is an industry need to perform effective viral clearance studies. I…

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