Search results for " affinity chromatography"
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Affinity chromatography Handbook: Vol. 2 Tagged Proteins
This handbook is intended for those interested in the expression and purification of recombinant proteins. The use of recombinant proteins has increased greatly in recent years, as has the wealth of…
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Protein L affinity resin is ready to move to manufacturing
Although protein L affinity resins have been available for more than a decade, insufficient binding capacities and alkaline stability challenges have limited their use in productive manufactur…
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Evaluation of Performance of a Disposable mAb Affinity Chromatography Column Used Over Multiple Process Cycles
Presanitized and prequalified disposable chromatography columns are widely used to save time in downstream processes. However, reuse of such columns could positively impact process economy. This app…
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Affinity Chromatography Handbook: Vol. 1 Antibodies
The diversity of the antibody-antigen interaction and our ability to manipulate the characteristics of the interaction has created many uses for antibodies and antibody fragments, both for immunoche…
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Case Study: Virus Reduction of an Affinity Capture Step
Evaluating how much a process step contributes to viral clearance is an essential part of process validation. For this reason, there is an industry need to perform effective viral clearance studies. I…
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Making the Move to Continuous Chromatography
Making the Move to Continuous Chromatography
Early adopters are benefiting from lower costs and increased productivity.
By Cynthia A. Challener
Tashatuvango/Shu…
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Improving Process-Scale Chromatography
She points to several new types of affinity chromatography resins introduced to the market in recent years that target recombinant plasma proteins, gene therapy applications, and different types of an…
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Process Chromatography: Continuous Optimization
“Protein-A based affinity chromatography is very popular and more widely used because it typically affords purities above 95% in one step,” observes Nandu Deorkar, vice-president of research and devel…
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Process Chromatography Selection for Downstream Processing Applications
By contrast, immobilized metal affinity chromatography is not often used because tagged proteins are uncommon in process scale.
Becerra-Arteaga (MilliporeSigma): Ion-exchange chromatography is mos…
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Innovative Chromatography Resins Can Improve Purity and Quality
From the early steps of downstream processing, affinity chromatography is already working to push through high purity product, which puts less stress on subsequent purification steps, he says. However…