Search results for " affinity" in Articles / App Notes

Article A Look at the Affinity Chromatography Landscape
Specific binding interactions between biologic molecules and immobilized affinity ligands allow for selective separations. With the advent of next-generation biopharmaceuticals, chromatography materia…

Article Affinity Chromatography Handbook in 3 Volumes
The Affinity Chromatography Handbook from Cytiva is divided into three volumes: Affinity Chromatography, Vol. 1: Antibodies Affinity Chromatography, Vol. 2: Tagged Proteins Affinity Chro…

Article Webinar about a new affinity chromatography technology for recombinant proteins
During a simple affinity step, the protein self-cleaves from the tag and elutes with no residual tag amino acids. The protocol does not require the use of a protease, and the same buffer can be used f…

Article Antibody Fragments and Their Purification by Protein L Affinity Chromatography
In the case of recombinant full-length antibodies with suitable Fc regions, two or three column purification processes centered around Protein A affinity chromatography have proven to be fast, efficie…

Article Bind, Cut, Elute — Easy Affinity Tag and Protease Removal
The tag is cleaved while the protein is still bound to the affinity resin. 

Article Efficient Cleaning-In-Place Methods for Protein-Based Antibody Affinity Chromatography Resins
As the use of sodium hydroxide (NaOH) is considered standard in the bioprocessing industry, the impact of treatment of affinity resins with various concentrations of this cleaning agent is demonstrate…

Article Affinity chromatography Handbook - Vol. 3: Specific Groups of Biomolecules
This volume of the Affinity Handbook describes the role of AC in the purification of specific groups of biomolecules, the principle of the technique, the chromatography media available and how to sele…

Article Protein L affinity resin is ready to move to manufacturing
Although protein L affinity resins have been available for more than a decade, insufficient binding capacities and alkaline stability challenges have limited their use in productive manufactur…

Article Case Study: Virus Reduction of an Affinity Capture Step
In this study, the virus reduction of an affinity capture step was evaluated in a scale-down column format. The study was performed by a third-party viral testing laboratory in collaboration with a bi…

Article Affinity chromatography Handbook: Vol. 2 Tagged Proteins
This handbook is intended for those interested in the expression and purification of recombinant proteins. The use of recombinant proteins has increased greatly in recent years, as has the wealth of…

Show All Results

Next Page