Search results for " HIC"

Article A Q&A With Prof. Alois Jungbauer: The Role of HIC in Biotech
What is your view of HIC in the biotech industry today and its role in the future? HIC is a powerful purification technique that is well established and will remain important in the future. It pro…

Tip Streamlining Screening: Hydrophobic interaction chromatography (HIC)
With the availability of 96-well formats, HIC can be approached in a systematic way, improving your chance of success and allowing you to apply high-throughput PD methods in your project. For details,…

Poster High-throughput screening of HIC media in PreDictor™ plates for capturing a recombinant protein from E.coli
A parallel screening of HIC media and conditions in 96-well plates facilitates the selection of the most promising HIC media and shows how salt type and salt concentration conditions could be tailored…

Article Increasing Productivity in Hydrophobic Interaction Chromatography (HIC) Using Capto™ Resins
In this study, the hydrophobicities of newer-generation Capto™ HIC resins were compared to Sepharose™ High Performance and Sepharose Fast Flow HIC resins. An application example is also presente…

Poster Novel HIC polishing platform with enhanced productivity
Modern downstream processes require high-productivity chromatography media (resins) allowing for shorter process time and increased window of operation, for an overall improved process economy.  A cha…

Article High-throughput screening of HIC media in PreDictor™ plates for capturing recombinant Green Fluorescent Protein from E. coli
This study demonstrates a rapid, high-throughput process development workflow using parallel screening in 96-well plates to determine the most suitable HIC media and conditions for capturing recombina…

Article Development of Purification for Challenging Fc-Fusion Proteins
In this study, both CEX and HIC resins were evaluated in a flow-through mode as a polishing step in purification of Fc-fusion proteins that previously proved challenging to purify using conventional A…

Article Addressing the challenges in downstream processing today and tomorrow
Anion exchange, cation exchange, and HIC resins were screened in 96 well plates in an HTS format. Additionally, ceramic hydroxyapatite (CHA), immobilized metal affinity chromatography (IMAC), and HIC

Article Process Chromatography: Continuous Optimization
Polishing is then achieved using some combination of anion exchange (AEX), cation exchange (CEX), mixed mode, and HIC resins. “Protein-A based affinity chromatography is very popular and more wide…

Article Tackling Analytical Method Development for ADCs
Early development of cytotoxicity assays and chromatographic assays such as HIC, reduced reverse phase high-performance liquid chromatography (RPHPLC), and SEC is therefore important. She adds that ad…

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