Search results for " HIC"

Article A Q&A With Prof. Alois Jungbauer: The Role of HIC in Biotech
What is your view of HIC in the biotech industry today and its role in the future? HIC is a powerful purification technique that is well established and will remain important in the future. It pro…

Article New hydrophobic interaction chromatography (HIC) handbook
Hydrophobic interaction chromatography (HIC) separates biomolecules under mild conditions according to differences in molecular hydrophobicity. HIC is often used as a next step to purify prote…

Article Increasing Productivity in Hydrophobic Interaction Chromatography (HIC) Using Capto™ Resins
In this study, the hydrophobicities of newer-generation Capto™ HIC resins were compared to Sepharose™ High Performance and Sepharose Fast Flow HIC resins. An application example is also present…

Article Optimization of a HIC step for recombinant protein purification
Follow this step-by-step high-throughput process development (HTPD) workflow to determine the optimal resin and initial conditions for hydrophobic interaction chromatography (HIC). …

Article Development of Purification for Challenging Fc-Fusion Proteins
In this study, both CEX and HIC resins were evaluated in a flow-through mode as a polishing step in purification of Fc-fusion proteins that previously proved challenging to purify using conventional A…

Article Addressing the Challenges in Downstream Processing Today and Tomorrow
Anion exchange, cation exchange, and HIC resins were screened in 96 well plates in an HTS format. Additionally, ceramic hydroxyapatite (CHA), immobilized metal affinity chromatography (IMAC), and HIC

Article Process Chromatography: Continuous Optimization
Polishing is then achieved using some combination of anion exchange (AEX), cation exchange (CEX), mixed mode, and HIC resins. “Protein-A based affinity chromatography is very popular and more wide…

Article Tackling Analytical Method Development for ADCs
Early development of cytotoxicity assays and chromatographic assays such as HIC, reduced reverse phase high-performance liquid chromatography (RPHPLC), and SEC is therefore important. She adds that ad…

Article Evolution of the Monoclonal Antibody Purification Platform
Mixed-mode resins based on ion exchange along with multimodal interactions can offer single-step operation as an alternative to two-step process of ion exchange and HIC. One such example is Capto Adhe…

Article Enhancing Resins Addresses Purification Concerns
“Additionally, these HIC and AEX resins are known to foul when used as a capture step, and alkaline stability of resins should be considered, such as resins based on polymethacrylate (e.g., Bakerbond …

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