Search results for " Fab"

Article Single-Domain Antibodies for Brain Targeting
IgG, the main serum antibody, the intact form of which is almost exclusively used in therapeutic antibodies, is a Y-shaped, multidomain protein with antigen-binding sites located on the two Fab (antig…

Article Modeling the Degradation of mAb Therapeutics
Cleavage of the peptide bond (hydrolysis) at the mAb’s hinge region leads to the formation of Fc -Fab and Fab species (22).  Responses from different classes of the mAb molecules vary with resp…

Article Antibody Production in Microbial Hosts
Figure 1: Structure of an antibody and its fragments: A) Antibody (IgG class); B) antibody fragments (VH, scFv, Fab, scFab, dAb, scFv-CH3). Abbreviations are: variable heavy chain (VH), variable light…

Article Addressing the Challenges in Downstream Processing Today and Tomorrow
Cimzia and Lucentis are two recently approved Fabs produced in E. coli (29) and although no scFv has yet received regulatory approved, there are a large number of both types of Ab fragments in clinica…

Article Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars A major advantage of SPR-based analysis is its ability to estimate the association and dissociation rate constants, an …

Article N-Glycan Analysis of Biotherapeutic Proteins
α-gal has been associated with IgE-mediated hypersensitivity reactions to Erbitux (cetuximab) produced in the mouse myeloma line SP2/0, where there are α-gal N-glycans on the Fab region (22). CHO cell…

Article Cost Considerations Drive Lean Technology in Biopharmaceutical Manufacturing
The Fab region of an antibody interacts with the target, while the Fc is engaged in cell-mediated cytotoxicity. Under EMA regulations, in-vitro studies must show that the antibody binds to the tar…

Article Resolving a bottleneck in screening and characterization of recombinant antibody fragments using Biacore 4000
Chimeric chicken/human Fab fragments were screened and characterized using a preoptimized Human Fab Capture Kit to achieve accurate off-rate determination during initial screening of crude samples. Th…

Article Get to know: mAb variants - eLearning
The diversity of antibody variants in the biotherapeutics pipeline are increasing with a growing interest for molecules like antibody fragments and bispecific antibodies (bsAbs). Cytiva has p…

Article Platform for Purification of VHH-type Antibody Fragments
However, it is known that protein A can also bind to VH3 sequences located on the variable heavy chain of the Fab region. This poster shows examples of purification of mAb-derived fragments.

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