Search results for " recombinant protein"

Article Re-use of Protein A Resin: Fouling and Economics
Mar 01, 2015 By Anurag S. Rathore, Mili Pathak, Guijun Ma, Daniel G. Bracewell BioPharm International Volume 3, Issue 28 In the past two decades, Protein A affinity chromatography ha…

Article Optimization of Protein Expression in Escherichia Coli
There are many parameters that affect cell growth and recombinant protein expression, such as choice of medium, carbon source, temperature, pH, aeration, inducer concentrations, and length of inductio…

Article Development of Purification for Challenging Fc-Fusion Proteins
Development of Purification for Challenging Fc-Fusion Proteins This study outlines methods for an alternative protein-polishing process for challenging proteins. …

Article Host-Cell Protein Measurement and Control
During the recombinant protein production, host cells also coproduce proteins related to the normal cell functions such as cell growth, proliferation, survival, gene transcription, protein synthesis, …

Article Fusion Proteins Pose Manufacturability Challenges
Making proteins ‘druggable’ Many proteins have short half-lives in human serum, making them unsuitable as recombinant protein drugs. When constructed as fusion proteins, however, they may become ‘…

Article Implications of Cell Culture Conditions on Protein Glycosylation
The choice of host-cell line for recombinant protein production depends first on the protein’s molecular properties. Certain bacteria can be used for production of the simplest proteins, those that ar…

Article Challenges of Protein Aggregation During Purification
…itor inclusion body solubilization, protein refolding, and aggregation near the production line of a recombinant protein-based vaccine candidate (1). Use of such real-time process analytical techniqu…

Article Platform Technologies Improve Protein Expression
“In the rapidly growing market of recombinant proteins and antibodies, our STEP technology provides a tool to complete preclinical phases at higher speed with reduced costs and with a higher success r…

Article Comparing Protein A Resins for Monoclonal Antibody Purification
The gene for Protein A has been cloned and expressed in Escherichia coli (6, 7) allowing for the production of large quantities of recombinant Protein A. Although recombinant Protein A is widely u…

Article Capture of human single-chain Fv (scFv) fusion protein on Capto L affinity medium
Capto L comprises a high-flow agarose base matrix to which recombinant protein L (E. coli) has been immobilized. A laboratory-scale purification step developed previously means that some parameters, e…

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