Search results for " CHO"
Article
Platform Approach Speeds Process Development
An expression platform has demonstrated fast-track development of high-quality, high-titer processes for producing biopharmaceuticals from CHO cells. The platform includes vector design, cell line eng…
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N-Glycan Analysis of Biotherapeutic Proteins
…a and ADCC activity (15), contrasting with the lower activity of mostly fucosylated mAbs produced in CHO cells. Consequently, glycoengineereing approaches have been developed to generate afucosyl ant…
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State of the Biopharmaceutical Industry: Lessons from 2018 Survey Data
Despite diverse genetic-engineering methods and host organisms being available, industry has clearly come to prefer and standardize on use of mammalian systems, particularly CHO. Many more efficient o…
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Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
In this study, the monoclonal antibody-expressing CHO DG44 cell line was used under license from Cellca GmbH, Laupheim, Germany.
Chemical analyses
Chemical analyses were performed by Toxikon E…
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Leveraging Computational Models of Glycosylation for Biopharma QA
• Tandem α-1,3 galactose is a non-human glycosylation motif that is produced by murine cell lines, such as NS0 and Sp2/0 (9) and has also been observed in CHO cells (10). Presence of α-1,3 galactose r…
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Development of Purification for Challenging Fc-Fusion Proteins
Development of Purification for Challenging Fc-Fusion Proteins
This study outlines methods for an alternative protein-polishing process for challenging proteins.
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Simple Fed-Batch Process Development for a Knockout Cell Line
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This workflow improved titers by 500% compared with Chinese hamster ovary cell culture in basal medium.
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News: Cytiva Adds ELISA Kit to End-to-End HCP Monitoring Capabilities
Cytiva is pleased to announce the new Amersham HCPQuant CHO kit, a generic for detecting host cell protein impurities in drug samples produced in CHO cell lines.
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Perfusion Culture Using TFF or ATF as Cell Retention Method
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Perfusion processes have many advantages such as being favorable for product quality and supporting efficient facility utilization by offering the possibility…
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Optimizing Late-Stage and Commercial Cell-Culture Processes
Whether these systems have the potential to disrupt the CHO hegemony for production of other proteins remains to be seen,” Racher says.
“These changes will eventually be at the level of the exp…