Search results for "purification"

Article Purification Strategies to Process 5 g/L Titers of Monoclonal Antibodies
Blank ABSTRACT Monoclonal antibody purification processes have been challenged recently to recover 5 g/L cell culture titers in existing manufacturing facilities. This requires the modificatio…

Article The Development and Application of a Monoclonal Antibody Purification Platform
The following article describes the development of a purification platform for monoclonal antibodies (MAbs) at Pfizer's Global Biologics division, including how the individual unit operations have bee…

Article Downstream scale-up purification of influenza virus using ReadyToProcess equipment
Total process time for the downstream purification was 3 days and the overall scale-up process resulted in production of influenza virus with the same yield and purity as obtained in the laboratory sc…

Article Economic drivers and trade-offs in antibody purification processes
Farid, PhD  ABSTRACT Increasing titers in mammalian cell culture has recently shifted the focus of process development efforts toward improving the economics of product recovery and purification

Article Development of Purification for Challenging Fc-Fusion Proteins
Monoclonal antibody purification processes exist in different well-established platforms with extensive process performance histories for production of commercial mAbs (6-10), which typically consist …

Article Comparing Protein A Resins for Monoclonal Antibody Purification
As a consequence, downstream processing must accept and handle higher titers of mAbs in harvested cell-culture fluid (HCCF), and vendors of mAb purification technologies must develop chromatography re…

Article A platform approach for the purification of antibody fragments (Fabs)
The high purification factor and generic conditions associated with this approach have proven particularly attractive to biopharmaceutical manufacturers. Antibody fragments, however, have previously l…

Article High-throughput process development and scale-up of an intermediate purification step for recombinant insulin
In purification development workflows, such HTPD methods have led to significant efficiency gains seen as a reduction in both the time and amount of sample required for selecting chromatographic media…

Article Challenges of Protein Aggregation During Purification
Importantly, the conditions that can lead to aggregation can occur during purification processes and need to be avoided or mitigated. “In particular, resolubilization of insoluble aggregates typically…

Article Evolution of the Monoclonal Antibody Purification Platform
This 31st article in the “Elements of Biopharmaceutical Production” series focuses on evolution of the purification platform for manufacturing of mAb therapeutics. Traditional Mon…

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