Search results for " sec"

Article SEC in the Modern Downstream Purification Process
While SEC may have mostly been abandoned in monoclonal antibody (mAb) and other protein purifications, it is still commonly used in the purification of viruses and plasmids. SEC, also known as gel…

Article Analyzing Proteins Using SEC, MALS, and UHPLC
Today, SEC is predominantly used to measure aggregation, because the determination of protein molecular weight by calibrating the retention times against those of known molecular standards is not effe…

Poster Optimal configuration of ÄKTA™ pure 25 for small-scale SEC
The cue card Optimal configuration of ÄKTA™ pure 25 for small-scale SEC describes how to improve results when performing SEC with sample volumes from 10 μl to 500 μl for analysis or small-scale p…

Article Analytical SEC: How Does Back Pressure Relate to Resolution?
Let’s straighten out what really helps you achieve high-resolution analytical SEC data. Read paper

Article Ensure SEC Column Reproducibility in Protein Drug Analysis
Size exclusion chromatography (SEC) is the go-to analytical method in the analysis of therapeutic protein drugs. This article describes the factors affecting the reproducibility of columns and how you…

Article Selectivity in Size Exclusion Chromatography (SEC): It’s Much More than Average Pore Diameter (Å)
Describing the pore of a size exclusion chromatography resin with a single number such as average pore diameter is an oversimplification. The formation of pores is dependent on complex combinations of…

Article Protein Characterization Using SEC: Three Webinar Takeaways
The possibility to separate molecules by size under native conditions is powerful in protein analysis. With more than thirty years in protein science, Åke Danielsson, Research Director at GE H…

Article Detecting Protein Aggregates and Evaluating their Immunogenicity
Users can add a light-scattering detector in line after SEC for irreversible aggregates, notes Some, and batch (unfractionated) light scattering methods can be applied to reversible aggregates. Analyt…

Article Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
Therapeutic monoclonal antibodies (mAbs) represent a rapidly growing area in the biopharmaceutical sector (2). Enormous success achieved with mAbs can be attributed to their distinctive beneficial pro…

Article HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
We recently reported a practical SEC method for injecting samples onto the column in rapid succession and gating the detection window to monitor the elution of each sample individually.27 Generally, s…

Next Page