Search results for " cell lines"

Article A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
By Luhong He, Christopher Frye Abstract The Chinese hamster ovary (CHO) cell lines used for the production of recombinant therapeutic proteins are immortalized cells with a relatively high degre…

Article Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
Therefore, applicability of this technique is restricted to a limited number of cell lines. Thermoresponsive substrates have been used to promote the detachment of adhered cells. In this method, t…

Article Changing the Culture of Cell Culture: Applying Best Practices and Authentication to Ensure Scientific Reproducibility
…ilieu, taking immediate steps to address biological reagent issues--specifically the use of cultured cell lines--is a relatively straightforward fix that will improve the credibility, reproducibility…

Article Best Practices for Selecting a Top-Quality Cell Line
Defining high-quality cell lines A high-quality cell line will demonstrate high stability, scalability, and high titer, so that it can provide reproducible results—with all product quality attribu…

Article The Evolving Role of Starting Materials in Cell and Gene Therapy
The Evolving Role of Starting Materials in Cell and Gene Therapy Accelerated approval pathways and growing demand for cell and gene therapies are putting pressure on providers of cellular starting m…

Article Commercial Production of Gene Therapies Using Suspension or Adherent Cell Lines
Adherent cell lines are the most common choice at early stages of development as they are readily sourced, easy to cultivate at the laboratory scale, and require less expert bioengineering know-how. …

Article Mapping a Route for Cell and Gene Therapy Process Development
He points in particular to the avoidance of the use of plasmids and the development of packaging/stable cell lines as current key issues. Reducing manufacturing costs, as well as development of…

Article Design Considerations for a Commercial Cell and Gene Therapy Facility
The commercialization of cell and gene therapies has become a reality, prompting deeper considerations of logistics, technology, and design for manufacturing facilities. By Feliza Mirasol …

Article Implications of Cell Culture Conditions on Protein Glycosylation
The authors present a review of the techniques commonly used for glycosylation analysis. By Michiel E. Ultee, PhD, Dr. Richard Easton This article reviews the implications of cell-culture co…

Article FDA Seeks to Enhance Manufacturing of Cell and Gene Therapies
He acknowledged that the logistics of manufacturing autologous cells can be particularly challenging, and that limited capacity in cell lines stymie efforts to meet the demand for product development.…

Next Page