Search results for " fragment"
Article
Witnessing Major Growth in Next-Generation Antibodies
The global market for next-generation antibodies, including antibody drug conjugates (ADCs), engineered antibodies, bispecific antibodies, antibody fragments, antibody-like proteins and biosimilar ant…
Article
A Look at the Affinity Chromatography Landscape
They have also needed to develop resin chemistries more suitable for the growing range of protein-based biologics, such as fusion proteins and antibody fragments, and affinity ligands that can separat…
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Implications of Cell Culture Conditions on Protein Glycosylation
Next, electrospray–mass spectrometry (ES–MS) can be used to fragment the permethylated glycans. This division action is useful, as the fragmentation pathways are well defined, well understood, and pro…
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Impact of Media Components on CQAs of Monoclonal Antibodies
The oxidized form of cysteine also reduces HMW and fragmentation. During cell culture, starvation of asparagine has been shown to result in misincorporation of serine during translation (11). Serine h…
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N-Glycan Analysis of Biotherapeutic Proteins
Immunoglobulin G (IgG) molecules consist of two heavy and two light chains, and the heavy chains contain an N-linked glycosylation site at asparagine 297 within the fragment crystallizable (Fc) region…
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Improving Process-Scale Chromatography
… that target recombinant plasma proteins, gene therapy applications, and different types of antibody fragments.
Thermo Fisher has introduced a new platform technology with large-scale production …
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Predicting Progress in Protein Aggregation
Targeted electron-transfer dissociation (ETD) fragmentation of the peptides that exhibit deuterium uptake provides further detailed information. “Additional information on the dynamics of these intera…
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Evolution of the Monoclonal Antibody Purification Platform
Major Adaptations to the mAb Platform
Use of multiple washes in Protein A
To understand the resolution of fragments and aggregates on Protein A, a pH gradient elution between pH 5.0 and 3.0 over 1…
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Top Tips for Successful Development of Antibody Chromatography Processes
We see more fragments, such as Fabs, scFv fusion proteins, domain antibodies, single chain fragments variable. And we also see bi- and multi-specific antibodies where you have variable site that can t…
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Get Started: Purification of mAb Variants - eLearning
Purification of bsAbs and antibody fragments isn’t as straightforward as for mAbs. These molecules show wider variation in binding affinities and are often more challenging to separate from impurities…