Search results for " filtration"

Article Selecting the Right Viral Clearance Technology
Ideally, methods from the first category, which include inactivation mechanisms such as low pH, detergent, or temperature hold steps and robust removal steps, such as virus filtration that clear a bro…

Article Viral Clearance Challenges in Bioprocessing
In addition to treatment with heat and ultraviolet radiation, virus retentive filtration is recognized as one of the most efficient and robust virus removal steps, and implementation in the media feed…

Article Challenges of Protein Aggregation During Purification
“All unit operations including chromatography, viral inactivation, viral filtration, and other filtration modes can create conditions that lead to protein aggregation, including shifts in pH and condu…

Article QbD and PAT in Upstream and Downstream Processing
Pressure and filter volume represent CPPs for viral filtration steps. Again, if engineering controls permit tight control of these parameters, some may be downgraded to a lower parameter designation. …

Article Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
The process was performed according to set specifications for a tangential flow filtration (TFF) process, with the added benefits of the single‑use technology and consistency through automation. …

Article Best Practices in Qualification of Single-Use Systems
Single-use buffer filtration system and storage bag application is common in downstream processes. Final drug-product sterile filtration and transfer using SUS has also being increasingly applied to f…

Article Using Single-Use Technologies in Downstream Processing
Some examples are filtration systems, buffer preparation systems, and sampling/dispensing systems. Key advantages of using single-use systems are speed of implementation, which is crucial for high-imp…

Article Trends in Downstream Bioprocessing
Depth filtration areas, by contrast, after hitting a low of 7.2% reporting “significant” or “severe” constraints, appear to have risen again, to 12% of respondents this year. Similarly, problems of th…

Article The Development and Application of a Monoclonal Antibody Purification Platform
After harvest, the cells were removed from the cell culture broth by depth filtration. In some cases, the broth was concentrated before the first chromatography step because of facility fit issues. Pr…

Article Automated Concentration and Diafiltration of Multiple siRNA Samples
Manual crossflow filtration (CFF) steps of the process lacked process robustness and were a source of variation between production runs. The project objective was to streamline the process to improve …

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