Search results for " host cell protein"
Article
Impurity Testing of Biologic Drug Products
Some impurities (especially host cell proteins) may add an immune-stimulating or adjuvant behavior to the therapeutic, causing the patient to generate antibodies or cell-mediated immunity against the …
Article
Re-use of Protein A Resin: Fouling and Economics
Specificity of Protein A ligand towards mAb results in extremely effective clearance of host cell proteins (HCP), viruses, and DNA (2–5). The high selectivity is accompanied by reasonably good physioc…
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Development of Purification for Challenging Fc-Fusion Proteins
As tested on three Fc-fusion proteins, this process demonstrates high process yield, improved clearance of HMWs and LMWs, and efficient reduction of host cell proteins (HCP) and DNA as well as robust …
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Antibody Production in Microbial Hosts
The authors review the status of expression of antibodies in microbial hosts and present the recent advances in the production of aglycosylated antibodies in bacteria.
By Anurag S. Rathore, Jyot…
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Platform Technologies Improve Protein Expression
Platform technologies facilitate development and accelerate commercialization of protein therapeutics.
By Cynthia A. Challener
Speed to market is essential in the biopharmaceutica…
Article
Quality by design for biotechnology products—part 1
Examples of process-related impurities include host cell DNA, host cell protein, adventitious agents, residual medium supplements such as protein hydrolysates, and components such as nucleases or resi…
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
Purity was determined by measuring host cell DNA (quantitative PCR), host cell proteins (Biacore method), and total protein (Bradford method).
The results in terms of TCID50, HA yield, host…
Article
Gene Therapies Push Viral Vector Production
Gene Therapies Push Viral Vector Production
Viral vectors show promise as a delivery mechanism for gene therapy, but which virus types are commercially viable?
By Feliza Miraso…
Article
Platform Approach Speeds Process Development
A modular cell-culture platform demonstrates accelerated process development.
By Harald Bradl, Jan Bechmann, Benedikt Greulich, Markus Michael Mueller, Patrick Schulz, Thomas Wucherpfennig
C…
Article
Fermentation for the Future
Improving efficiency, value chain, quality, and protein complexity with advanced bioprocess development.
Jan 01, 2015
By Cynthia Challener, PhD
Microorganisms—bacteria and yeast—have b…