Search results for " size exclusion chromatography"
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Many of the recommended protein characterization assays are based on liquid chromatography methods such as ion exchange chromatography (IEC) for charge heterogeneity analysis, size exclusion chromatog…
Process Chromatography Selection for Downstream Processing Applications
Industry experts discuss best practices for selecting a separation technology. By Caroline Hroncich Process chromatography has changed significantly since it was first introduced to the biop…
Virus purification using superheated buffer in place (SBIP) for packed bed chromatography
Superheated buffer in place (SBIP) is a method for sterilizing packed chromatography beds used to process viruses and biomolecules that are too large for sterile filtration. Superheating occurs when…
Teaching the Basics of Chromatography
Have you ever come across the need to teach others about the basics oh chromatography? Maybe new hires would benefit from learning about the principles of different chromatography techniques. In a s…
Selection Guide Size Exclusion Chromatography Columns and Media
Size exclusion chromatography (SEC), also called Gel filtration (GF), separates molecules on the basis of differences in size as they pass through a SEC medium packed in a column. A variety of media…
Protein Analysis with Size Exclusion Chromatography
Size exclusion chromatography (SEC) is widely used in several different analytical applications from basic research to quality control of biotherapeutics. This white paper presents fundamentals abou…
Size exclusion chromatography analysis of papain-cleaved monoclonal antibody using Superdex™ 200 Increase columns
This application note describes a simplified workflow for the production of Fab (fragment, antigen binding). Fab was produced by papain cleavage of a purified monoclonal antibody (MAb) followed by pur…
New Size Exclusion Chromatography Handbook Available!
GE´s popular SEC handbook has recently been updated with • A new structure, including a new chapter on analytical SEC • The latest Superdex™Increase and Superose™Increase columns are now incl…
Your Guide to Chromatography Media
A selection guide to chromatography methods: Affinity chromatography; size exclusion chromatography, and ion exchange chromatography.
Handbook: Size Exclusion Chromatography Principles and Methods
This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection crite…