Search results for " detection"
Article
Detecting Protein Aggregates and Evaluating their Immunogenicity
…rough bonds that can be broken by ionic processes, means that we need to rely on both separation and detection,” which makes it especially difficult.
Aggregation risk factors
Certain manufact…
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
Mass spectrometry (MS) can be used to establish the identity of the drug (primary sequence) and for detection of size-distribution profiles linked to post-translational modifications such as glycosyla…
Article
Western Blot Detection: Is Film or a Digital CCD Imager Better?
It is based on building an antibody:protein complex via specific binding of antibodies to proteins immobilized on a membrane and detecting the bound antibody with one of several detection methods.
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Article
Current Trends in Host Cell Protein Detection for Biologics Manufacturing
Commonly accepted HCP analytical methods, the challenges of HCP assay, and recommendations on what can easily be accomplished in-house and when it may be better to outsource.
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Host-Cell Protein Measurement and Control
The detectability of residual HCPs, however, also depends on the method of detection’s sensitivity. Industry addresses this risk by meticulous method development and the use of multiple technologies t…
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Real Time Continuous Microbiological Monitoring
LIF is extremely sensitive, with a limit of detection down to one microbial cell.
Real-time microbial monitoring using LIF
The use of Trypticase Soy Agar (TSA), also known as Tryptone Soya Aga…
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Microbiological Testing: Time is of the Essence
Conventional protocols no longer suitable for traditional biologics
Microbiology testing of pharmaceutical products is performed with a view to detection, enumeration, and identification of microbi…
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An Integrated Approach to Ensure the Viral Safety of Biotherapeutics
The goal of SbD is to protect a manufacturing process from interruptions caused by viral contamination and ensure product and patient safety by preventing virus introduction, ensuring early detection,…
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A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
The assay was shown to be specific for the detection of glucose in all studied multi-component media without matrix interference regardless of glucose concentration or the vendor.
In the past few …
Article
Ensuring Viral Safety of Viral Vaccines and Vectors
Beyond these tests, other techniques for the detection of retroviruses may be implemented. Retrovirus particles can be detected using transmission electron microscopy (TEM). Reverse transcriptase enzy…