Search results for " TFF" in Articles / App Notes
Article
Perfusion Culture Using TFF or ATF as Cell Retention Method
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Perfusion processes have many advantages such as being favorable for product quality and supporting efficient facility utilization by offering the possibility…
Article
Downstream Processing for Cell-Based Therapies
However, this technique, although suitable for the purposes of the study, is not a scalable technique like TFF. Other groups have used TFF and expanded-bed chromatography (EBC) as continuous, integrat…
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Exosome isolation by tangential flow filtration and size exclusion chromatography
We found that:
- Cytiva™ superSEC resin enriched exosomes from impurities compared to legacy Sepharose™ CL-2B resin—a well-established resin for exosome purification—after the initial TFF step.
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Article
Single-Use for Downstream Chromatography: Benefit or Hindrance?
As Young points out, reusable systems can typically require more than 12 hours from set-up to tear-down, while single-use TFF systems require half that time.
Another area where single-use is gainin…
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Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
This work describes the capabilities of ÄKTA readyflux when used in ultrafiltration/diafiltration (UF/DF) applications. The process was conducted in an automated manner based on methods created …
Article
Process Chromatography: Continuous Optimization
A salt-tolerant resin can be very beneficial to a purification process by decreasing the need for dilution or eliminating TFF steps all together,” she notes.
“Next-generation IEX resins that offer…
Article
Unifying Continuous Biomanufacturing Operations
…angential-flow filtration (TFF), comments Morbidelli, although he says the scale-up for both ATF and TFF remains, to a certain extent, an open issue. Bonham-Carter notes that using ATF for cell reten…
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Downstream Processing Continues to Worry CMOs
Indeed, a majority of CMOs are considering using single-use disposable TFF membranes and single use-prepacked columns. It remains to be seen the extent to which these will help. Downstream continues t…
Article
Tools for Continuous Bioprocessing Development
External hollow-fiber systems such as the ATF and TFF couple hollow-fiber filters to a conventional bioreactor. These systems circulate cells between the conventional bioreactor and the hollow fibers …
Article
Increasing mRNA capabilities and capacity for growing market needs
The rapid development of innovative vaccines allowed for the pharmaceutical industry to deliver its first tools in the fight against the COVID-19 pandemic.
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