Search results for "protein binding" in Articles / App Notes
Article
How To Interpret Protein Binding Capacity: Chromatography Resins
Explore what protein binding capacity is, how it can be measured and what exactly to look for on a manufacturer’s specification sheet in this blog post.
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Article
Alkaline stability of Fc and VH3 binding in protein A resins
In the development of antibody purification protocols, you need to know your Ab molecule and its possible affinity binding sites for the chromatography resins. In this study, we have looked at…
Article
How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins
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In protein purification, dynamic binding capacity (DBC) of a chromatography column describes the maximum amount of target protein that you can load onto your co…
Article
Interpreting Protein Binding Capacities for Chromatography Resins
In this post, we explain what protein binding capacity is, how it can be measured, and what to look for on a manufacturer's specification sheet.
Article
Dynamic Binding Capacity in Fiber-Based Chromatography
Fiber-based matrices for affinity purification is suitable for rapid-cycling chromatography. Fiber chromatography matrices such as Fibro PrismA can bind a large amount of protein in a frac…
Article
Bind, Cut, Elute — Easy Affinity Tag and Protease Removal
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This blog post discusses automated methods for removing an affinity tag and the protease. The tag is cleaved while the protein is still bound to the affinity re…
Article
Re-use of Protein A Resin: Fouling and Economics
Mar 01, 2015
By Anurag S. Rathore, Mili Pathak, Guijun Ma, Daniel G. Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography ha…
Article
Optimization of Protein Expression in Escherichia Coli
May 01, 2015
By Siavash Bashiri, David Vikström, Nurzian Ismail
BioPharm International
Volume 28, Issue 5
Production of proteins for manufacturing therapeutics and pharmaceuticals is a c…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Newer classes of biotherapies will require innovations in processing technology.
By Glen R. Bolton, Bernard N. Violand, Richard S. Wright, Shujun Sun, Khurram M. Sunasara, Kathleen Watson, Johna…
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
Surface plasmon resonance is helping define bispecific antibodies, the next-generation of biopharma therapeutics.
By Robert Karlsson
Abstract
Biotherapeutic antibodies are still the larg…